Czech Republic (the)
Faculty of Science, Charles University| The award | How you will study | Study duration | Course start | Domestic course fees | International course fees |
|---|---|---|---|---|---|
| PhD | Full-time | find out | find out | find out | find out |
Human cells contain three eIF4E protein isoforms encoded by separate genes. Each is capable of binding to the 5' mRNA cap and exists in several variants resulting from alternative mRNA splicing. All eIF4E isoforms play important roles in development, cancer and cellular response to stress. Cellular level and activity of the canonical eIF4E1 are tightly regulated by mTOR and Mnk kinases. Overexpression of eIF4E1 can lead to cellular transformation and indeed up to one third of human cancers show an increase in eIF4E1 levels. In addition to its well-recognized role in mRNA silencing and translation in hypoxia, the non-canonical eIF4E2 is one of the proteins whose deregulation creates part of the signature of metastatic cells. In contrast, eIF4E3 has been proposed to act as a tumor suppressor protein. The aim of the proposed project is to use advanced pull-down and chromatography methods followed by LC-MS to purify, analyze and characterize active protein complexes in which the non-canonical members of the eIF4E family occur in human cancer cells, mouse oocytes and early embryos. We offer an enthusiastic team, a well-equipped and well-funded laboratory and close international collaboration with some laboratories at EMBL, Heidelberg.
The candidate should have an expertise in biochemistry and/or molecular biology
Five relevant publications of the research group (lab members are underlined):
Iyyappan, R., Aleshkina, D., Ming, H., Dvoran, M., Kakavand, K., Jansova, D., Del Llano, E., Gahurova, L., Bruce, A. W., Masek, T., Pospisek, M., Horvat, F., Kubelka, M., Jiang, Z. & Susor, A. The translational oscillation in oocyte and early embryo development. Nucleic Acids Res (2023), doi:10.1093/nar/gkad996.
del Llano, E., Masek, T., Gahurova, L., Pospisek, M., Koncicka, M., Jindrova, A., Jansova, D., Iyyappan, R., Roucova, K., Bruce, A. W., Kubelka, M. & Susor, A. Age-related differences in the translational landscape of mammalian oocytes. Aging Cell 19 (2020), doi:10.1111/acel.13231.
Frydr
Contact Faculty of Science, Charles University to find course entry requirements.
Below are some suggested courses at other providers that you may also be interested in:
Clinical and Developmental Psychopathology (Research Master) Master Degree
Vrije Universiteit Amsterdam
Find out more
Distance and Independent Studies Center, University of Kaiserslautern-Landau (RPTU), Germany
Find out more
Psychology Bachelor of International Liberal Arts
International College of Liberal Arts (iCLA) at Yamanashi Gakuin University
Find out more
Art and Design - Advanced Master Masters by Research
KdG University of Applied Sciences and Arts
Find out moreIf you do not meet the entry requirements for this course then consider one of these postgraduate preparation courses from another institution:
Graduate Diploma of Engineering (Civil: Structural)
Engineering Institute of Technology
Find out more
There are 136 other courses listed from Faculty of Science, Charles University. A selection of these are displayed below:
A postdoctoral position to study thermal effects in landslides Postdoc
Faculty of Science, Charles University
Find out more Activation of STING signaling in tumors associated with human papillomaviruses PhD
Faculty of Science, Charles University
Find out more Advanced Electron Microscopy Techniques for the Characterization of Tunable Zeolitic Architectures PhD
Faculty of Science, Charles University
Find out more Amphiphilic Peptidomimetics Containing Metallacarboranes as a Design Strategy for Future Antibiotics PhD
Faculty of Science, Charles University
Find out more See other universities in Prague
Find out more about studying in Czech Republic (the)
